Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background Mus musculus | C57Bl/6J | Charles River | ||
| Genetic reagent Mus musculus | Hoxb1GFP | PMID:11076756 | RRID:MGI:1928090 | Mario Capecchi (HHMI) |
| Genetic reagent Mus musculus | Hoxb1IRES-Cre | PMID:12815623 | RRID:MGI:2668513 | Mario Capecchi (HHMI) |
| Genetic reagent Mus musculus | Tg(CAG-Hoxb1-EGFP)1Sza | PMID:30134070 | ||
| Genetic reagent Mus musculus | Mef2c-AHF-Cre | PMID:15253934 | RRID:MGI:4940068 | Brian Black (UCSF) |
| Genetic reagent Mus musculus | Gt(ROSA)26Sortm1Sor(R26R) | PMID:9916792 | RRID:MGI:1861932 | |
| Genetic reagent Mus musculus | Gt(ROSA)26Sortm9(CAG-tdTomato)Hze | PMID:20023653 | RRID:MGI:3809523 | |
| Genetic reagent Mus musculus | Mlc1v-nlacZ-24 or Fgf10Tg(Myl3-lacZ)24Buck | PMID:15217909 | RRID:MGI:3629660 | |
| Genetic reagent Mus musculus | Mlc3f-nlacZ-2E or Tg(Myl1-lacZ)1Ibdml | PMID:15217909 | RRID:MGI:5449224 | |
| Gene (Mus musculus) | Hoxb1 | Mus musculus genome resource | RRID:MGI:96182 | |
| Antibody | Rabbit anti-GFP Polyclonal Antibody | Thermo Fisher Scientific | Cat# A-11122, RRID:AB_221569 | IF (1:500) |
| Antibody | Rabbit anti-Hoxb1 Polyclonal Antibody | Covance | Cat# PRB-231P-100, RRID:AB_291592 | IF (1:200) |
| Antibody | Mouse anti-αactinin Monoclonal Antibody | Sigma | A7732, RRID:AB_2221571 | IF (1:500) |
| Antibody | Mouse anti-myosin Monoclonal Antibody | DHSB | Cat# MF 20, RRID:AB_2147781 | IF (1:100) |
| Antibody | Rabbit anti- cleaved Caspase3 Polyclonal Antibdoy | Cell signaling Technology | Cat# 9661, RRID:AB_2341188 | IF (1:300) |
| Antibody | Rabbit anti-phospho-Histone H3 Polyclonal Antibody | Millipore | Cat# 06–570, RRID:AB_310177 | IF (1:400) |
| Antibody | Mouse anti-Islet1 Monoclonal Antibody | DSHB | Cat# 39.4D5, RRID:AB_2314683 | IF (1:100) |
| Antibody | Rabbit anti-Tbx1 Polyclonal Antibody | LifeSpan | Cat# LS-C31179-100, RRID:AB_911118 | IF (1:100) |
| Antibody | Goat anti-Tbx5 Polyclonal Antibody | Santa Cruz | Cat# sc-17866, RRID:AB_2200827 | IF (1:250) |
| Antibody | Donkey anti-rat IgG (H+L) Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21208, RRID:AB_141709 | IF (1:500) |
| Antibody | Donkey anti-Mouse IgG (H+L) Alexa Fluor 555 | Thermo Fisher Scientific | Cat# A-31570, RRID:AB_2536180 | IF (1:500) |
| Antibody | Goat anti-rabbit IgG (H+L) Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-21244, RRID:AB_2535812 | IF (1:500) |
| Sequence-based reagent | mm-Hoxb1-C1 Mus musculus | Acdbio | 541861 | RNA-FISH probe |
| Sequence-based reagent | mm-Nppa Mus musculus | Acdbio | 418691 | RNA-FISH probe |
| Sequence-based reagent | mm-Bmp4-C1 Mus musculus | Acdbio | 401301 | RNA-FISH probe |
| Sequence-based reagent | mm-Gata4 Mus musculus | Acdbio | 417881 | RNA-FISH probe |
| Sequence-based reagent | mm-Aldh1a2 Mus musculus | Acdbio | 447391 | RNA-FISH probe |
| Sequence-based reagent | mm-eGFP-C3 | Acdbio | 400281 | RNA-FISH probe |
| Sequence-based reagent | mm-Isl1 Mus musculus | Acdbio | 451931 | RNA-FISH probe |
| Sequence-based reagent | mm-Nr2f2-C3 Mus musculus | Acdbio | 480301 | RNA-FISH probe |
| Sequence-based reagent | mm-tdTomato-C3 | Acdbio | 317041 | RNA-FISH probe |
| Sequence-based reagent | mm-Tbx1-C1 Mus musculus | Acdbio | 481911 | RNA-FISH probe |
| Sequence-based reagent | mm-Tbx5-C2 Mus musculus | Acdbio | 519581 | RNA-FISH probe |
| Sequence-based reagent | mm-Osr1-C2 Mus musculus | Acdbio | 496281 | RNA-FISH probe |
| Commercial assay or kit | RNAscope Multiplex Fluorescent v2 Assay | Acdbio | 323110 | |
| Commercial assay or kit | Ovation RNAseq v2 kit | NuGEN | 7102–08 | RNA-seq |
| Commercial assay or kit | AffinityScript Multiple Temperature cDNA synthesis kit | Agilent | 600107 | cDNA synthesis kit |
| Commercial assay or kit | Master Mix PCR Power SYBR Green | ThermoFisher | 4334973 | qPCR |
| Commercial assay or kit | NucleoSpin RNA XS | Machery-Nagel | 740902.50 | RNA extraction |
| Recombinant DNA reagent | pNppa(−633/+87)-Luc (plasmid) | PMID:12023302 | Vincent Christoffels (Amsterdam University Medical Center) | |
| Recombinant DNA reagent | pTnnT2(−497/+1)-Luc (plasmid) | PMID:12023302 | Vincent Christoffels (Amsterdam University Medical Center) | |
| Cell line (Cercopithecus aethiops) | Cos-7 | ATCC | RRID:CVCL_0224
CRL-1651 |
|
| Cell line Mus musculus | NIH3T3 | ATCC | RRID:CVCL_0594
CRL-1658 |
|
| Cell line Mus musculus | mESTet-On/Hoxb1 | PMID:18499896 | Anthony Gavalas (Technische Universitat Dresden) | |
| Sequence-based reagent | Bmp4-FW | This paper | qPCR primers | TTCCTGGTAACCGAATGCTGA |
| Sequence-based reagent | Bmp4-Rev | This paper | qPCR primers | CCTGAATCTCGGCGACTTTTT |
| Sequence-based reagent | Bag3-Fw | This paper | qPCR primers | GCCCTAAGGACACTGCATCTT |
| Sequence-based reagent | Bag3-Rev | This paper | qPCR primers | GCTGGGAGTAGGCATGGAAA |
| Sequence-based reagent | Bak1-Fw | This paper | qPCR primers | CCTTCTGAACAGCAGGTTGC |
| Sequence-based reagent | Bak1-Rev | This paper | qPCR primers | GACCCACCTGACCCAAGATG |
| Sequence-based reagent | Dapk1-Fw | This paper | qPCR primers | GAGGTGGTGGCTGCGTC |
| Sequence-based reagent | Dapk1-Rev | This paper | qPCR primers | CGCAGACCTCCGGTCC |
| Sequence-based reagent | GFP-Fw | This paper | qPCR primers | CGACGTAAACGGCCACAAGTT |
| Sequence-based reagent | GFP-Rev | This paper | qPCR primers | TTGATGCCGTTCTTCTGCTTGT |
| Sequence-based reagent | Hoxa1-Fw | This paper | qPCR primers | AGAAACCCTCCCAAAACAGG |
| Sequence-based reagent | Hoxa1-Rev | This paper | qPCR primers | TTGTTGAAGTGGAACTCCTTCTC |
| Sequence-based reagent | Hoxb1-Fw | This paper | qPCR primers | AAGAGAAACCCACCTAAGACAGC |
| Sequence-based reagent | Hoxb1-Rev | This paper | qPCR primers | TGAAGTTTGTGCGGAGACC |
| Sequence-based reagent | Hprt-Fw | This paper | qPCR primers | TGTTGGATATGCCCTTGACT |
| Sequence-based reagent | Hprt-Rev | This paper | qPCR primers | GATTCAACTTGCGCTCATCT |
| Sequence-based reagent | Isl1-Fw | This paper | qPCR primers | GCAACCCAACGACAAAACTAA |
| Sequence-based reagent | Isl1-Rev | This paper | qPCR primers | CCATCATGTCTCTCCGGACT |
| Sequence-based reagent | Nppa-Fw | This paper | qPCR primers | CACAGATCTGATGGATTTCAAGA |
| Sequence-based reagent | Nppa-Rev | This paper | qPCR primers | CCTCATCTTCTACCGGCATC |
| Sequence-based reagent | Nppb-Fw | This paper | qPCR primers | GTCCAGCAGAGACCTCAAAA |
| Sequence-based reagent | Nppb-Rev | This paper | qPCR primers | AGGCAGAGTCAGAAACTGGA |
| Sequence-based reagent | Nr2f2-Fw | This paper | qPCR primers | CCTCAAAGTGGGCATGAGAC |
| Sequence-based reagent | Nr2f2-Rev | This paper | qPCR primers | TGGGTAGGCTGGGTAGGAG |
| Sequence-based reagent | Osr1F-Fw | This paper | qPCR primers | AGAAGCGTCAGAAGTCTAGTTCG |
| Sequence-based reagent | Osr1R-Rev | This paper | qPCR primers | GGAACCGCAATGATTTCAA |
| Sequence-based reagent | Parp1-Fw | This paper | qPCR primers | AGGGCTGCCTGGAGAAGATA |
| Sequence-based reagent | Parp1-Rev | This paper | qPCR primers | TCGTCCCGCTTCTTGACAAA |
| Sequence-based reagent | Raldh2-Fw | This paper | qPCR primers | CATGGTATCCTCCGCAATG |
| Sequence-based reagent | Raldh2-Rev | This paper | qPCR primers | GCGCATTTAAGGCATTGTAAC |
| Sequence-based reagent | Tbx5-Fw | This paper | qPCR primers | CCCGGAGACAGCTTTTATCG |
| Sequence-based reagent | Tbx5-Rev | This paper | qPCR primers | TGGTTGGAGGTGACTTTGTG |
| Sequence-based reagent | Trp53-Fw | This paper | qPCR primers | AGTATTTCACCCTCAAGATCCGC |
| Sequence-based reagent | Trp53-Rev | This paper | qPCR primers | GGAGCTAGCAGTTTGGGCTT |