Figure 1. FHL1 expression was downregulated in several BC tissues and BC cell lines. (A) FHL1 expression in various BC tissues was analyzed via StarBase. (B) A Kaplan-Meier plotter was used to analyze the correlation between FHL1 expression and survival rate of patients with BC. (C) FHL1 expression in TNBC tissues and adjacent breast tissues was determined using qRT-PCR. (D) FHL1 expression in the normal human breast epithelial cell line MCF-10A and human BC cell lines (SK-BR-3, BT549, MCF7, MDA-MB-231, and MDA-MB-453) was determined using qRT-PCR. GAPDH was used as an internal control. The data are shown as mean ± standard deviation.

BC = breast cancer; FHL1 = four and a half LIM protein 1; TNBC = triple-negative breast cancer; qRT-PCR = quantitative real-time reverse transcription polymerase chain reaction; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.

^*p < 0.5 or ^†p < 0.001 vs. cancer tissue. ^‡p < 0.5 or ^§p < 0.01 or ^∥p < 0.001 vs. MCF-10A.