Figure 3. The transfection of FHL1 or siFHL1 regulated invasion and tube length of BT549 and MDA-MB-231 cells. (A and B) Invasion of BT549 cells transfected with siFHL1 or FHL1 was determined using a Transwell invasion assay. (C and D) The invasion rate in MDA-MB-231 cells transfected with siFHL1 or FHL1 was determined using a Transwell invasion assay. (E and F) The tube length of BT549 cells transfected with siFHL1 or FHL1 was determined using a tube formation assay. (G and H) The tube length of MDA-MB-231 cells transfected with siFHL1 or FHL1 was determined using a tube formation assay. The data are shown as mean ± standard deviation.

FHL1 = four and a half LIM protein 1; siFHL1 = siRNAs specifically targeting FHL1; NC = negative control; siNC = siRNA negative control.

^*p < 0.01 or ^†p < 0.001 vs. siNC. ^‡p < 0.001 vs. NC.