Table 1. Primer sequences used for qRT-PCR.
| Genes | Primer sequences (5′-3′) | |
|---|---|---|
| miR-183-5p | ||
| forward | TATGCCACTGCTAGA ATTCACT | |
| reverse | GTGCACGGTCCGACGT | |
| FHL1 | ||
| forward | TGCTGCCTGAAATGCTTTGAC | |
| reverse | GCCAGAAGCGGTTCTTATAGTG | |
| VEGF | ||
| forward | AGGGCAGAATCATCACGAAGT | |
| reverse | AGGGTCTCGATTGGATGGCA | |
| p53 | ||
| forward | CAGCACATGACGGAGGTTGT | |
| reverse | TCATCCAAATACTCCACACGC | |
| E-cadherin | ||
| forward | CGAGAGCTACACGTTCACGG | |
| reverse | GGGTGTCGAGGGAAAAATAGG | |
| N-cadherin | ||
| forward | TCAGGCGTCTGTAGAGGCTT | |
| reverse | ATGCACATCCTTCGATAAGACTG | |
| Vimentin | ||
| forward | GACGCCATCAACACCGAGTT | |
| reverse | CTTTGTCGTTGGTTAGCTGGT | |
| GADPH | ||
| forward | GGAGCGAGATCCCTCCAAAAT | |
| reverse | GGCTGTTGTCATACTTCTCATGG | |
| U6 | ||
| forward | CTCGCTTCGGCAGCACA | |
| reverse | ACGCTTCACGAATTTGCGT | |
qRT-PCR = quantitative real-time reverse transcription polymerase chain reaction; FHL1 = four and a half LIM protein 1; VEGF = vascular endothelial growth factor; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.