Fig. 1.

Construction diagram of recombinant baculovirus (rBV) and identification of cVLPs. A The ectodomain of NDV F protein was replaced with that of AIV H9 protein (H9/F). DCpep sequence was fused to the amino terminal of H9/F protein through a GS-linker and co-expressed with NDV HN protein on the surface of NDV VLPs. B, C Negative staining TEM of H9/F-cVLPs and DCpep-H9/F-cVLPs. D, E, F, G Western blot analysis of cVLPs through mouse anti-NDV and anti-H9N2 polyclonal serum. The molecular weight (MW) of M, H9/F, DCpep-H9/F and HN were approximately 40, 65, 65 and 68 kDa, respectively.