Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 1;10:14349. doi: 10.1038/s41598-020-71453-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HBV is internalized via the EGFR endocytosis pathways. (A) The dose-dependent EGFR endocytosis pathway modulated by EGF. The illustration of EGFR monomer and dimer were referred to previous report²³. (B,D,F) Relative HBV infection levels in HepG2-NTCP cells (−), HepG2-NTCP cells treated with ikarugamycin (IKA) (B), filipin (FLP) (D) and chloroquine (CQ) (F). The results are shown as the mean ± SEM of 3 independent experiments. *p < 0.05. **p < 0.01. ***p < 0.001. N.S., not significant. (C) Immunofluorescence staining for EGFR (green) and NTCP (Red) in HepG2-NTCP cells at 2 ng/ml of EGF treated with or without 2 µM IKA. Scale bar, 10 µm. (E) Immunofluorescence staining for EGFR (green) and LAMP2 (Red) in HepG2-NTCP cells at 50 ng/ml of EGF treated with or without 1 µM FLP. Scale bar, 10 µm. (F) Immunofluorescence staining for EGFR (green) and LAMP2 (Red) in HepG2-NTCP cells at 2 ng/ml and 50 ng/mL of EGF treated with 25 µM CQ. Scale bar, 10 µm.