Table 1.
Genes regulated in the head after mating are enriched in genes linked to wound-healing, hemocytes and the JNK pathway.
| Gene name | AGAP | Mating regulation | Link to melanization28,29 | Upregulated by wounding27 | Hemocyte-enriched32 |
|---|---|---|---|---|---|
| Upregulated genes | |||||
| Melanization | |||||
| PPO2 | AGAP006258 |
2.5-fold, 24 hpm p = 0.0082 |
✓ | ✓ | |
| PPO5 | AGAP012616 |
1.8-fold, 24 hpm p = 0.0065 |
✓ | ✓ | |
| PPO6 | AGAP004977 |
2.1-fold, 24 hpm p = 0.0070 |
✓ | ✓ | |
| CLIPB1 | AGAP003251 |
1.7-fold, 3 hpm p = 0.012 |
✓ | ✓ | |
| CLIPB15 | AGAP009844 |
2.8-fold, 24 hpm p = 0.011 |
✓ | ✓ | ✓ |
| CLIPC7 | AGAP003689 |
2.1-fold, 24 hpm p = 0.00049 |
✓ | ✓ | ✓ |
| CLIPE11-like | AGAP003691 |
3.8-fold, 3 hpm p = 0.000018 |
✓ | ✓ | ✓ |
| Gastrulation-defective | AGAP013252 |
1.2-fold, 24 hpm p = 0.0035 |
✓ | ||
| Serpin 17 | AGAP001376 |
1.8-fold, 3 hpm p = 0.0016 |
✓ | ✓ | |
| Yellow F2 | AGAP004324 |
2.4-fold, 3 hpm p = 0.0075 |
✓ | ✓ | |
| TEP/LRIM family | |||||
| TEP1 | AGAP010815 |
3.1-fold, 24 hpm p = 0.0034 |
✓ | ✓ | |
| TEP4 | AGAP010812 |
1.7-fold, 24 hpm p = 0.00049 |
✓ | ✓ | |
| TEP8 | AGAP010831 |
3.3-fold, 24 hpm p = 0.0012 |
✓ | ||
| TEP19 | AGAP010832 |
3.3-fold, 24 hpm p = 0.0035 |
✓ | ||
| APL1C | AGAP007033 |
2.1-fold, 24 hpm p = 0.018 |
✓ | ✓ | ✓ |
| Coagulation | |||||
| TGase2 | AGAP009098 |
2.4-fold, 3 hpm p = 0.0095 |
✓ | ✓ | ✓ |
| Other | |||||
| membrane protease | AGAP001365 |
1.5-fold, 3 hpm p = 0.0078 |
✓ | ||
| Unknown | AGAP004316 |
3.6-fold, 3 hpm p = 0.0075 |
|||
| Vesicle transport | AGAP006609 |
1.1-fold, 3 hpm p = 0.0084 |
|||
| Carboxylsterase | AGAP011509 | 2.2-fold, 3 hpm p = 0.0029 | |||
| Hsc70 | AGAP004192 | 2.5-fold, 3 hpm p = 0.017 | |||
| Hsp90b | AGAP001424 | 1.1-fold, 3 hpm p = 0.012 | |||
| Down regulated genes | |||||
| Acyltransferase | AGAP007078 |
1.5-fold, 3 hpm p = 0.012 |
|||
The 23 genes identified by microarray as being significantly regulated in the head after mating were compared with the relevant literature reports indicated. The mean mating-induced fold-change over age-matched virgins, the time point at which that change was observed and the adjusted p value, after False Discovery Rate (FDR) correction for multiple testing, are indicated (further detail of statistical validation of microarray data is included in “Methods”).