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. 2020 Sep 1;10:14386. doi: 10.1038/s41598-020-71362-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Increased SF CD19⁺CD24^hiCD27⁺ B cells were inversely correlated with PB CD19⁺CD24^hiCD27⁺ B cells in patients with RA. Representative flow cytometry charts depicting the gating strategy for CD19⁺CD24^hiCD27⁺ B cells (P3) in healthy PB (a). The percentage of PB CD19⁺CD24^hiCD27⁺ B cells was analyzed in 26 patients with RA, 16 patients with OA and 20 healthy controls (HC) (b). The proportion of SF CD19⁺CD24^hiCD27⁺ B cells was assessed by flow cytometric analysis in 26 RA and 16 OA patients (c). The correlations between the percentage of SF CD19⁺CD24^hiCD27⁺ B cells and paired PB CD19⁺CD24^hiCD27⁺ B cells of RA patients (n = 26) (d) and OA patients (n = 16) (e) were evaluated respectively. ***p < 0.001; NS, not significant.