Table 1.
Primers and probes used in this study.
| Target | References | Gene target | Nucleotide sequence 5′–3′ | Limit of detection |
|---|---|---|---|---|
| Dreissena spp. | Gingera et al.16 | 16 s | DRE16SF TGGGGCAGTAAGAAGAAAAAAATAA | 1 copy |
| DRE16SR CATCGAGGTCGCAAACCG | ||||
| DRE16SP FAM/CCGTAGGGATAACAGC/MGBNFQ | ||||
| Naegleria spp. | Sheehan et al.12 | ITS | NF GAACCTGCGTAGGGATCATTT | NA |
| NR TTTCTTTTCCTCCCCTT ATTA | ||||
| O. nerka | Sepulveda et al.7 | COI | SSF CTGCCCTTCTCCTTACGATTT | 1 copy |
| SSR CAGTGGATCAGAGGAGTGTTAG | ||||
| SSP AATCGCCATCCTGTTCCTCCTGTT | ||||
| S. japonicus | Sassoubre et al.15 | COI | 324F GCTGAACAGTTTATCCTCCCCTCG | 0.1 pg/μL |
| 430R CCCAAGGATTGAGGAAACACCTGCTAG | ||||
| 349P FAM/TGGGAACCTGGCACACGCCGGG/BHQ | ||||
| T. byrosalmonae | Hutchins et al.13 | 18 s | 1337F CGAACGAGACTTCTTCCTT | 7 copies |
| 1426R CTTCCTACGCTTTTAAATAGCG | ||||
| 1399P FAM/CCCTTCAATTAGTTGATCTAAACCCCAATT/IBFQ |