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. 2020 Jun 12;123(5):730–741. doi: 10.1038/s41416-020-0906-7

Fig. 2. Ibuprofen suppresses cancer cell metastasis and stemness in vivo.

Fig. 2

a Schema of the metastasis model established by subcutaneous implantation of 4T1-luci cells into the fourth pair of mammary fat pad of BALB/c mice. b Tumour growth curve of 4T1-luci with or without ibuprofen treatment. c The body weight of BALB/c mice in the course of ibuprofen treatment. d The survival curve of BALB/c mice inoculated with 4T1-luci with or without ibuprofen treatment. e The representative luciferase images showing the 4T1-luci tumours at the primary site and lung metastasis sites with or without ibuprofen treatment. f Representative H&E staining images of 4T1-luci tumour metastasis to the lung with or without ibuprofen treatment, scale bar: 50 µm. g Statistic results of metastasis loci of 4T1-luci tumour metastasis to the lung with or without ibuprofen treatment. h Immunohistochemistry staining of SOX2 and OCT4 in 4T1-luci primary tumours with or without ibuprofen treatment. Representative images with 20× magnification were shown, scale bar: 50 µm. i Statistic results of SOX2- or OCT4-positive cells in 4T1-luci primary tumours with or without ibuprofen treatment. j Schema of the chemoresistance model established by subcutaneous implantation of 4T1-luci cells into the fourth pair of mammary fat pad of BALB/c mice. k Tumour growth curve of 4T1-luci with or without ibuprofen treatment in the presence of cisplatin. l The body weight of BALB/c mice in the course of ibuprofen treatment in the presence of cisplatin. m The survival curve of BALB/c mice inoculated with 4T1-luci with or without ibuprofen treatment in the presence of cisplatin. n The representative luciferase images showing 4T1-luci tumours at the primary sites with or without ibuprofen treatment on day 7 (before cisplatin administration) and day 18 (after cisplatin administration). o Immunohistochemistry staining of SOX2 and OCT4 in 4T1-luci primary tumours with or without ibuprofen treatment in the presence of cisplatin, scale bar: 50 µm. Representative images with 20× magnification were shown. p Statistic results of SOX2- or OCT4-positive cells in 4T1-luci primary tumours with or without ibuprofen treatment in the presence of cisplatin. All data shown are representative of three experiment repeats. q Representative images showing TUNEL staining of tumour tissue from each group. Scar bar, 100 μm.