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. 2020 Jun 12;123(5):730–741. doi: 10.1038/s41416-020-0906-7

Fig. 4. Ibuprofen mediates histone 3 methylation and acetylation to affect target genes’ expression in vitro and in vivo.

Fig. 4

a Western blot examining the expression of histone 3 modification markers, KDM6A, KDM6B and HDACs in A549, MDA-MB-231 and HepG2 cells with or without ibuprofen treatment. b Immunofluorescence staining of H3 modification markers in A549, MDA-MB-231 and HepG2 cells with or without ibuprofen treatment, scale bar: 10 µm. c CHIP-qPCR detecting the expression of ICAM3 DNA fragment in A549, MDA-MB-231 and HepG2 cells with or without ibuprofen treatment. d HDAC activity in A549, MDA-MB-231 and HepG2 cells with or without ibuprofen treatment. e Immunohistochemistry staining examining the expression of H3 modification markers in 4T1-luci tumours separated from BALB/c mice. Representative images with 40× magnification were shown. f The bar graph shows the statistic results of H3 modification marker-positive cells. g CHIP-qPCR detecting the expression of ICAM3 DNA fragment in 4T1-luci tumours separated from BALB/c mice with or without ibuprofen treatment. All data shown are representative of three experiment repeats.