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. 2020 Jun 12;123(5):730–741. doi: 10.1038/s41416-020-0906-7

Fig. 5. Ibuprofen mediates H3 methylation and acetylation to regulate ICAM3 expression in vitro.

Fig. 5

a Western blot examining the COX1- and COX2-knockdown efficiency in A549 cells. b ALDH staining assay was performed to check ALDH + sub-population percentage in A549-shCtrl, shCOX cells with or without ibuprofen treatment. c Side-population assay was performed to detect SP percentage in A549-shCtrl, shCOX cells with or without ibuprofen treatment. d, e Statistic results of ALDH + subpopulation and side population are shown. f Western blot examining the expression of histone 3 modification markers, KDM6A, KDM6B and HDACs in A549-shCtrl, shCOX cells with or without ibuprofen treatment. g HDAC activity in A549-shCtrl, shCOX cells with or without ibuprofen treatment. h Western blot examining the expression of ICAM3 in A549-shCtrl, shCOX cells with or without ibuprofen treatment. All data shown are representative of three experiment repeats.