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. 2020 Sep 1;10:14390. doi: 10.1038/s41598-020-71345-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The relative expression levels of miR-210 in serum and visceral WAT from the agomir/ncRNA/Ctrl groups of 4,300 m-raised mice. (B) The relative expression levels of miR-92a in serum and visceral WAT from the agomir/ncRNA/Ctrl groups of 4,300 m-raised mice. The results were obtained by qPCR analysis. The relative quantification was obtained by the ∆∆CT method with normalization to U6. n = 6 per group. (C) Brown adipose-selective genes’ relative expression levels in BAT from the agomir/ncRNA/Ctrl groups of 4,300 m-raised mice. The relative quantification was obtained by the ∆∆CT method compared with 18S rRNA. n = 6 per group (*p ≤ 0.05 indicates statistical significance. **p ≤ 0.01, ***p ≤ 0.001, Student’s t test. The Ctrl-(4,300 m) group was used as the control.