Table 3.
Primer sequences for genes.
| mRNAs/miRNAs | 5′ → 3′ |
|---|---|
| 18S | |
| Forward | TTGACGGAAGGGCACCACCAG |
| Reverse | GCACCACCACCCACGGAATCG |
| Ucp-1 | |
| Forward | CAGAGGTCGTGAAGGTCAGAATGC |
| Reverse | GGACATCGCACAGCTTGGTACG |
| Ppargc1α | |
| Forward | CCGAAGACACTACAGGTTCCATAG |
| Reverse | GGGAGGGAGAGAGGAGAGAGG |
| Prdm16 | |
| Forward | CAACAAAGAGAAGCCGTTCAAG |
| Reverse | TTTCGGATCTCGGAGAAGTAAG |
| Pparα | |
| Forward | GAGCTGCAAGATTCAGAAGAAG |
| Reverse | GAATCTTTCAGGTCGTGTTCAC |
| Pparγ | |
| Forward | TGTGGACCTCTCCGTGATGG |
| Reverse | GGTTCTACTTTGATCGCACTTTGG |
| Nrf1 | |
| Forward | CCACGTTGGATGAGTACACG |
| Reverse | GCACCACATTCTCCAAAGGT |
| Tfam | |
| Forward | TCAGGAGCAGCAGGCACTACA |
| Reverse | CTGAGCTCCGAGTCCTTGAACAC |
| Tmem26 | |
| Forward | GTGGTGTGGACGTGGAGTATGC |
| Reverse | GTAGATCATCAGGACGAGGCGC |
| Hif-1α | |
| Forward | CCACCACAACTGCCACCACTG |
| Reverse | TGCCACTGTATGCTGATGCCTTAG |
| Fgfr-1 | |
| Forward | TTGCCAGACCAAGAAGAAGCCATG |
| Reverse | GTCCTTGTCACCACTGCGTTCTC |
| has-/mmu-miR-210-3p | |
| Forward | CUGUGCGUGUGACAGCGGCUGA |
| has-/mmu-miR-92a-3p | |
| Forward | UAUUGCACUUGUCCCGGCCUG |
| hsa-miR-155-5p | |
| Forward | UUAAUGCUAAUUGUGAUAGGGGU |
| hsa-miR-99a-5p | |
| Forward | AACCCGUAGAUCCGAUCUUGUG |
| has-miR-30c-5p | |
| Forward | UGUAAACAUCCUACACUCUCAGC |
| hsa-miR-32-3p | |
| Forward | CAAUUUAGUGUGUGUGAUAUUU |
| hsa-miR-185-5p | |
| Forward | UGGAGAGAAAGGCAGUUCCUGA |
| hsa-miR-130a-3p | |
| Forward | CAGUGCAAUGUUAAAAGGGCAU |