Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 1;10:14374. doi: 10.1038/s41598-020-71220-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assessment of spectral features of the NIR-excited fluorescence and its correlation with melanin-related Raman bands. (A) Illustration of the emission maximum estimation procedure. Fluorescence background was approximated by a 2^nd order polynomial, so the emission maximum could be calculated as a center of the parabola. (B) Representative spectra with high fluorescence (Fl-FP > 10) color-coded according to the position of the center of the parabola. (C) Typical Raman spectrum of the skin with high melanin content obtained for a volunteer with skin type III after sunlight exposure at a depth of 38 µm (stratum basale). Melanin twin peaks are denoted as dashed lines. (D) Correlation between the fluorescence emission maximum and the ratio of melanin Raman bands at 1,380 and 1,570 cm⁻¹.