Fig. 5. Transcripts associated with Notch signalling are upregulated in a subset of SCLC tumours expressing high levels of t-DARPP.

a Expression of t-DARPP transcripts was quantified using a previously published RNA-Seq database.³⁷ Blue points indicate individual SCLC patients (n = 6) with an elevated t-DARPP expression (t-DARPP: both DARPP-32 isoforms) in tumour tissue compared to mRNA derived from corresponding adjacent normal tissue. b Quantification of mRNA transcripts in a subset of SCLC patients (n = 6; blue points) expressing high level of t-DARPP isoforms in tumour relative to normal tissue. c Gene set enrichment analysis (GSEA) was performed to identify cellular pathways regulated in a subset of SCLC patients with elevated t-DARPP levels in tumour tissue. Notch signalling was enriched by GSEA. d Heat map showing the expression of Notch family member genes identified through GSEA. N and T denotes normal and tumour tissue, respectively. Red colour represents highest fold change in expression in tumour tissue relative to normal. Colour bar represents normalised read counts in a log scale.