Cell line |
Derived from agriculturally-relevant species
Capable of differentiation into meat-relevant cell types (muscle, fat, fibroblast, etc.)
Genetically stable and immortalized
Optimized for large-scale growth (tolerate suspension, controlled differentiation, etc.)
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Development of small molecule cocktails that can replace the need for genetic approaches to induce pluripotency and to facilitate maintenance of pluripotency
Footprint-free methods of cell line engineering using RNA or protein delivery or excisable transposons
Improved protocols for cell freezing to maintain viability and phenotypic fidelity
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Culture media |
Animal component-free, antibiotic-free, ideally chemically defined
Optimized for meat-relevant cell lines and co-culture of multiple cell types
Extremely low cost and high-volume production capacity
Engineered or synthetic growth factors
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Scaffolding |
Edible and/or biodegradable and food grade materials
Support cell adherence
Support vascularization and media perfusion
Biomechanical properties suitable for tissue maturation
Scalable production capacity
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Biocompatible, non-animal-derived scaffolding materials pioneered in the regenerative medicine field
Use of tunable scaffold parameters (stiffness, etc.) to spatially direct differentiation
Degradable materials that enable cell migration and vascularization after patient implantation
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Bioreactors |
Support cell proliferation as well as tissue maturation/perfusion
Large volume, low maintenance
High-yield cell harvesting
Real-time, in-line cell monitoring for quality control
Integrated media filtration and recycling system
Highly automated; closed system
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Integrated, closed systems with increasing automation to reduce errors and contamination risk associated with human handling
In-line monitoring of media components to adjust perfusion in real time
Novel technologies to improve efficiency of cell separation and harvesting
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