Figure 5. Detection of ROS in kidneys and HK-2 cells by DHE staining.

(A) Kidney tissue sections of mice receiving PBS, Fc-ELA (1 mg/kg) and/or LPS were stained with DHE and the fluorescence intensity was quantified by fluorescence microscopy. Quantification of ROS production was done by measuring fluorescence intensity. (B) HK-2 cells were pre-treated with PBS/albumin (Cont) or Fc-ELA (0, 5 μM) for 30 min and then with or without LPS (2 μg/ml) for 1 h. The cells were incubated with DHE for 20 min and quantified by microscopy. Quantification of ROS production was done by measuring fluorescence intensity. Data are expressed as mean + SE (n=9). ***P<0.001 and n.s., no statistical significance.