Fig. 7.

The effect of inhibition of clathrin‐independent endocytic routes on the cellular uptake of T‐Fc. U2OS‐R1 cells were subjected to siRNA‐mediated silencing of a μ2 subunit of the AP2 complex (AP2μ2), dynamin‐2 (DNM2), galectin‐3 (GAL3), ROCK1, and ROCK2. Cells were incubated for 15 min with 100 nm T‐Fc and fluorescently labeled transferrin as an internal control of CME inhibition. Internalized T‐Fc was visualized with Zenon AF‐488, and its co‐localization with immunolabeled EEA1 was determined with confocal microscopy. Scale bars represent 50 µm.