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. 2020 May 24;14(9):2231–2250. doi: 10.1002/1878-0261.12700

Fig. 11.

Fig. 11

linc‐ROR decreased the sensitivity of breast cancer cells to rapamycin through linc‐ROR/miR‐194‐3p/MECP2 regulatory axis. (A) CCK8 cell viability assay revealed that the overexpression of miR‐194‐3p group had lower cell viability than negative control and the rescue of miR‐194‐3p expression reduced the inhibiting effect of linc‐ROR on it. (B–E) Western blot assays determined the expression level of mTOR protein, pictures of protein bands were captured, and the gray value of them was calculated by Image J. (B, C) linc‐ROR downregulated the protein level of mTOR; (D, E) miR‐194‐3p upregulated the protein level of mTOR, and the rescue of miR‐194‐3p expression reduced the downregulating effect of linc‐ROR on it. (F) The correlation analysis of the expression level of MECP2 and mTOR from TCGA database. ‘miR‐194‐3p mimic/NC mimic’: overexpression of miR‐194‐3p/its negative control in MCF‐7 cell line, ‘LV‐linc‐ROR + miR‐194‐3p mimic/NC mimic’: rescue/not rescue the expression of miR‐194‐3p in linc‐ROR‐overexpressing MCF‐7 cell line; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; error bars represent SEM.