Figure 2.

Carba1 has a low cytotoxicity. (A) Effect of Carba1 on HeLa cell viability. Cells were incubated for 72 h with increasing concentrations of Carba1. The percentage of viable cells was calculated following the Prestoblue assay. The results are expressed as mean ± SEM of three separate experiments. (B) Effect of Carba1 on HeLa cells apoptosis. HeLa cells, treated with the indicated concentrations of Carba1 for 72 h, were stained with propidium iodide and FITC-annexin V and analyzed by flow cytometry. Apoptotic cells are observed in the upper right part of the graphs. (C) Results for apoptotic cell death (as shown in Figure 2B) are expressed as mean ± SEM of three separate experiments. The significance was determined by a Student’s t-test (** p < 0.01, compared to the control).