Figure 3.

Effects of CTN on MG132-induced apoptosis in GS hiNPCs. (A) MG132-induced apoptotic cell death and recovery by CTN treatment were evaluated through Annexin V/7-AAD staining. (B) Quantification of apoptotic cells by flow cytometry. (C) Cell lysates from CTN-treated hiNPCs immunoblotted with the apoptosis marker cCASP3. GAPDH was used as an internal control. (D) Quantification of Western blots for cCASP3 normalized over GAPDH. Data are mean ± standard error of means (SEM) of at least three independent experiments. p-values were analyzed using the unpaired two-tailed Student’s t-test (* p < 0.05, ** p < 0.005, *** p < 0.001).