Figure 6.

mRNA expression of NRF2-mediated cytoprotective molecules. Antioxidant molecules, such as (A) superoxide dismutase 1 (SOD1), (B) peroxiredoxin 1 (PRX1), (C) peroxiredoxin 5 (PRX5), (D) glutathione peroxidase 1 (GPX1), and (E) the phase II antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 (NQO1) were analyzed in WT hiNPCs, GS hiNPCs, and GS hiNPCs treated with CTN (3 μM) for 24 h followed by MG132 treatment (10 μM, 24 h). In addition, the mitochondrial biogenesis marker (F) peroxisome proliferator-activated receptor γ coactivator-1alpha (PGC1α) was analyzed. Data are mean ± standard error of means (SEM) of at least three independent experiments. p-values were analyzed using the unpaired two-tailed Student’s t-test (* p < 0.05, ** p < 0.005, *** p < 0.001).