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. 2020 Aug 3;9(8):1826. doi: 10.3390/cells9081826

Figure 3.

Figure 3

Increased EDB-FN expression in breast cancer cells with TGF-β treatment and drug resistance. ZD2-Cy5.5 staining of 3D cultures of breast cancer cells shows significantly increased EDB-FN expression in (A) MCF7-TGF-β and MCF7-DR and (B) MDA-MB-468-TGF-β and MDA-MB-468-DR cells, compared to the parent MCF7 and MDA-MB-468 cells, respectively. ZD2 peptide binding to EDB-FN was quantified in FIJI as the ratio of the pixel intensities of ZD2-Cy5.5 to that of Hoechst to show increased EDB-FN level in (C) MCF7-TGF-β and MCF7-DR and (D) MDA-MB-468-TGF-β and MDA-MB-468-DR cells, compared to the parent MCF7 and MDA-MB-468 cells. Bars denote mean ± sem and dots denote independent replicates. * p < 0.05 using Mann–Whitney U test. qRT-PCR analysis shows significantly upregulated mRNA expression of EDB-FN in (E) MCF7-TGFβ and MCF7-DR and (F) MDA-MB-468-TGFβ and MDA-MB-468-DR cells, compared to the parent MCF7 and MDA-MB-468 cells, respectively. Dots denote 3 technical replicates from 3 independent experiments, with lines at mean ± sem. * p < 0.05 using Mann–Whitney U test. Scale bars = 100 µm.