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. 2020 Aug 11;9(8):1885. doi: 10.3390/cells9081885

Figure 2.

Figure 2

Metabolic profile of different cybrids after hypoxia-ischemia. Comparison of ATP production (A), pyruvate dehydrogenase (PDH) activity (B), lactate dehydrogenase (LDH) activity (C) under hypoxic-ischemic condition in cybrid cells harboring stroke-susceptible haplogroup F and stroke-resistant haplogroup B. Measurements of oxygen consumption rate (OCR) under normoxic (D) and hypoxic (E) conditions, and comparison of the fundamental OCR parameters under normoxic (G) and hypoxic (H) conditions, in cybrid cells harboring stroke-susceptible haplogroup F and stroke-resistant haplogroup B. (F) represents the fundamental parameters of mitochondrial OCR including basal respiration (a), coupled respiration (b), uncouple respiration (c), maximal respiration (d), and spare respiratory capacity (e). Mitochondrial ATP levels, PDH and LDH activity were determined by the colorimetric assay kit. Data represent the mean ±SD of at least three independent experiments (* p < 0.05; # p < 0.005). N was used to represent normoxic and H to represent hypoxic condition. OCR examined by the Seahorse XF24 analyzer in the absence or presence of oligomycin (1 μM), FCCP (250 nM), and rotenone (1 μM) under normoxic or hypoxic conditions, data obtained by counting 1 × 105 cells of cybrids.