Figure 5.
TAK-242/BTZ treatment impaired the hexosamine biosynthetic pathway. (A) Concentrations of glycosylated UDP-derivatives were calculated by HPLC analysis in deproteinized cells 24 h post-treatment with BTZ, TAK-242 or their combination. Values are the mean of three different experiments and are expressed as nmol/106 cells. UDP-Gal = UDP-galactose; UDP-Glc = UDP-glucose; UDP-GalNac = UDP-N-acetylgalactosamine; UDP-GlcNac = UDP-N-acetylglucosamine. (B) The mRNA transcription of OGT and OGA (relative to B2M) was determined by RTqPCR after 24 h. Calculated value of 2^−ΔΔCt in untreated cells was 1. * p < 0.05; ** p < 0.01; *** p < 0.001 (p values refer to control or BTZ-treated cells).