Figure 1.

Overview of single-cell RNA sequencing (scRNA-seq) methodology. Single-cell RNA sequencing technology is used to explore transcriptomic profiles of single cells that are isolated from cell lines, organisms, or tissue/blood samples of clinical material. Massive datasets can be generated and analyzed by a specific algorithm that allows the discernment of cell-to-cell heterogeneity, lineage tracing, and stochastic gene expression at the single-cell level.