Figure 5.

Effect of high light (HL) or combined HL and cold on CA activity. (A) CA activity in extracts of WT, SALK_106570C, and SALK_131447 plants treated with HL or cold together with HL for 1 h. ΔβCA1 activity was calculated as difference between the WT CA activity and the mean activity of the two βca1 knock out lines from the corresponding treatment. Data represent the mean ± SE of n ≥ 8 from three independent experiments. Significant differences of p ≤ 0.05 are indicated by different letters a-e. Significance of difference was calculated using one-way ANOVA with post hoc Tukey HSD test. (B) Western blot with specific βCA1-antibody of treated WT plant material with and without mPEG-Mal labeling of free thiols.