Table 3.
Specific peroxidase activity of secreted metal chelate affinity chromatography (MCAC)-purified MoHyBPOX1 with various electron donors at each determined pH optimum, recorded at 30 °C. All values are means ± standard deviation. For comparison, standard reduction potentials (E0’ in (V)) for used electron donors obtained from the literature are given in the second column.
| Substrate | E0´ of Sub. (V) | Detection at WL (nm) | pH Optimum | Specific Activity (U/mg) |
|---|---|---|---|---|
| ABTS | 1.08 | 414 | 6.5 | 217.8 ± 8.3 |
| Guaiacol | 0.43 | 470 | 6.0 | 8.0 ± 1.1 |
| L-DOPA | 0.47 | 470 | 6.5 | 103.1 ± 6.6 |
| 5-Aminosalicylate | 0.54 | 470 | 6.5 | 188.4 ±10.3 |
| Catechol | 1.20 | 350 | 5.5 | 5.7 ± 0.8 |
| Resorcinol | 1.70 | 290 | 5.0 | 1.1 ± 0.4 |
| Pyrogallol | 0.56 | 430 | 5.5 | 40.2 ± 0.7 |
| 3,3,5,5-Tetramethylbenzidine | 0.60 | 652 | 4.5 | 21.1 ± 1.5 |
| Ascorbate | 0.28 | 290 | 6.0 | 7.2 ± 0.1 |
| Cytochrome c | 0.24 | 550 | 7.0 | 0 * |
| Mn2+ | 1.51 | 238 | 8.5 | 7.2 ± 0.3 |
| L-Tyrosine | 0.93 | fluorescence | 7.5 | 621.6 ± 39.2 FU ** |
* recorded via repeat scans compared with a control sample where Cytc was oxidized only non-enzymatically (i.e., scan-background); ** arbitrary fluorescence units.