Table 4.

Michaelis–Menten parameters of purified MoHyBPOX1 for selected electron donors *.

Electron Donor	KM (µM)	kcat (s−1)	kcat/KM (M−1 s−1)
ABTS	11.2 ± 1.0	106.3 ± 7.4	9.52 × 106
L-DOPA	69.7 ± 2.8	24.5 ± 5.4	3.51 × 105
Pyrogallol	29.3 ± 1.3	1.1 ± 0.2	3.62 × 104
Guaiacol	97.3 ± 1.9	0.5 ± 0.1	5.16 × 103
TMB	69.2 ± 0.7	0.2 ± 0.02	2.75 × 103
Ascorbate	250.2 ± 2.3	1.2 ± 0.2	4.92 × 103
Mn2+	762.4 ± 6.9	4.5 ± 0.3	5.96 × 103

* recorded at 30 °C, at respective pH optima of tested substrates.