Table 1.

Characterization of lethality and dysmorphogenesis in zebrafish embryos, produced by oxidative stress-related compounds.

Compounds	Range of Concentrations	MTC	LC50	EC50	Exposure Window
OS Inducers
Tert-butyl hydroperoxide (tBOOH)	1–4 mM	n.d.a	2.4 mM	1.6 mM	26–50 hpf
Tetrachlorohydroquinone (TCHQ)	2.5–20 µM	n.d.a	16.0 µM	3.9 µM	26–50 hpf
Lipopolysaccharides from Escherichia coli 0111:B4 (LPS)	5–60 µg/mL	25 µg/mL	50.1 µg/mL	35.9 µg/mL	26–50 hpf
Modulators of Antioxidant Status
n-acetyl-l-cysteine (NAC)	50–2500 µM	250 µM	1874 µM	920.6 µM	2–26 hpf
Diethyl maleate (DEM)	0.1–100 µM	0.5 µM	n.d.b	1.5 µM	2–26 hpf
Nω-nitro l-arginine methyl ester hydrochloride (L-NAME)	0.1–100 µM	5 µM	n.d.c	44.36 µM	2–26 hpf
DL-buthionine sulfoximine (BSO)	1–5000 µM	50 µM	n.d. c	2722 µM	2–26 hpf
Antioxidants
Vit. E	1–1000 µM	100 µM	n.d.d	n.d.d	2–26 hpf
Lipoic acid	0.1–1000 µM	5 µM	116.4 µM	n.d.c	2–26 hpf
Quercetin	0.1–30 µM e	20 µM	n.d.d	n.d.d	2–26 hpf

Range of tested concentrations, maximum tolerable concentration (MTC), lethal concentration 50 (LC₅₀), effective concentration 50 for dysmorphogenesis (EC₅₀) and exposure window for each of the studied compounds. n.d.: data were not determined. ^a: MTC was not determined because the compound produced lethal or dysmorphogenic effects at all the studied concentrations. ^b: LC₅₀ was not calculated because no lethal effects were observed until the highest concentration, where lethality was of 100%. ^c: LC₅₀ or EC₅₀ was not calculated because no significant effects in the mortality of the embryos were observed. ^d: LC₅₀ and EC₅₀ were not calculated because the compounds did not produce lethal or dysmorphogenic effects at any of the tested concentrations. ^e: Quercetin solution precipitated from 30 µM. It was not possible to evaluate the effects at higher concentrations.