Table 3.
Effects of antioxidant compounds in lethality and dysmorphogenesis of zebrafish embryos exposed to tBOOH.
| Antioxidant Compounds | OS Inducer | LC50 (95% CI) | EC50 (95% CI) |
|---|---|---|---|
| None 1 | Tert-butyl hydroperoxide (tBOOH) | 2.38 mM (2.28–2.48) |
1.64 mM (1.44–1.87) |
| Vitamin E | 2.83 mM *** (2.70–2.69) |
2.42 mM *** (2.17–2.70) |
|
| Lipoic acid | 3.72 mM *** (3.14–4.40) |
3.70 mM *** (3.03–4.51) |
|
| Quercetin | 3.26 mM *** (2.83–3.76) |
3.05 mM *** (2.64–3.54) |
Lethal concentration 50 (LC50), effective concentration 50 for dysmorphogenesis (EC50) and 95% confidence interval. Statistically significant differences with respect to the group which was not exposed to any antioxidant compound: ***: p < 0.001; 1 A unique tBOOH concentration–response curve was generated and compared to all the concentration–response curves of the antioxidants pre-exposure groups (initially dissolved or not in DMSO) due to the lack of effect of DMSO in the embryonic development of ZF.