Figure 2.

SRA-hLECs overexpressing Bmal1 showed augmented expression of Nrf2 and Nrf2/antioxidant response element (ARE)–dependent antioxidants. SRA-hLECs transfected with pGFP-Vector or pGFP-Bmal1 plasmid. Total RNA and protein were isolated from transfectants and qPCR and protein analysis were conducted as described in the Materials and Method’ section. Bmal1 (a) overexpression significantly increased Nrf2 (b), Prdx6 (c), NQO1 (d), SOD1 (e), and SOD2 (f) mRNA (A) and protein (B) expression. Protein blots were quantified using densitometer, and levels were normalized to corresponding GAPDH levels. Histograms are shown in right side of Western blot. Data represent means ± S.D. of three independent experiments. Violet vs. light orange and dark orange bars; *p < 0.05, **p < 0.001. #; Endogenous Bmal1.