Figure 1.

OAT-449, similarly to vincristine, strongly inhibits viability in multiple cancer cell lines. Live cells were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, in which 10⁴ cells were seeded in 96-well plates treated with OAT-449, vincristine or 0.1% DMSO as control and analyzed 72 h after treatment. Log(dose)–response curves for HT-29, HeLa, DU-145, Panc-1, SK-N-MC, SK-OV-3, MCF-7 and A-549 are shown as labeled. Each data point represents the mean ± SEM of at least 3 independent experiments. Each EC₅₀ was calculated based on sigmoidal curve fitting to the respective data set.