Figure 5.

Both OAT-449 and vincristine cause mitotic defects, multi-nucleation, aneuploidy and polyploidy through the blocking of the tubulin polymerization and induces G2/M phase cell cycle arrest. Cancer cells HT-29 and HeLa were grown on coverslips and treated with control culture medium, 30 nM OAT-449 or vincristine for 24 h, fixed with 2% formaldehyde, stained with DAPI and Alexa Fluor 488-conjugated anti-tubulin antibody (green fluorescence) and examined using confocal microscopy. The images are representative of at least 3 independent experiments.