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OAT-449 causes a reduction in phosphorylation of Cdk1 immunoblotting of P-Cdk1 (Tyr15) in whole cell lysate of HT-29 in basal condition and upon 18 h treatment with 30 nM OAT-449 or vincristine. (a) Immunoblot, showing biological duplicate lanes (1–2) for each condition. (b) Densitometry of immunoblot data, with signal intensity relative to β-tubulin (mean ± SEM). Data are representative of 3 independent experiments.
