Table 1.
Observed allele ranges and distribution of genotyping errors among microsatellite loci in historical samples of studied cattle populations (%).
| # | Locus | Observed Allele Ranges, bp a | Number of Alleles | Amplification Failure, % | ADO Rate, % | FA Rate, % | ER, % |
|---|---|---|---|---|---|---|---|
| 1 | BM2113 | 121–141 | 10 | 0.00 | 1.29 | 0.37 | 1.47 |
| 2 | BM1824 | 178–188 | 5 | 0.74 | 3.24 | 0.74 | 2.96 |
| 3 | ETH10 | 211–225 | 8 | 2.94 | 3.23 | 0.38 | 2.65 |
| 4 | ETH225 | 140–160 | 8 | 1.84 | 1.03 | 0.37 | 1.12 |
| 5 | INRA023 | 198–216 | 9 | 5.88 | 2.29 | 1.17 | 3.13 |
| 6 | SPS115 | 248–260 | 6 | 5.51 | 4.07 | 1.17 | 3.89 |
| 7 | TGLA122 | 139–181 | 16 | 0.74 | 1.99 | 1.11 | 2.59 |
| 8 | TGLA126 | 115–127 | 7 | 2.57 | 3.69 | 0.75 | 3.77 |
| 9 | TGLA227 | 77–103 | 12 | 0.74 | 0.90 | 1.11 | 1.85 |
| Mean value | 9.00 ± 3.35 | 2.33 ± 0.31 | 2.35 ± 0.36 | 0.79 ± 0.18 | 2.59 ± 0.33 | ||
Locus, microsatellite locus; observed allele ranges, the limits of allelic lengths of studied microsatellite loci in historical samples analysed (a allele sizes were standardised according to International Society of Animal Genetics (ISAG) International Bovine (Bos Taurus) short tandem repeat (STR) typing comparison test 2018–2019); amplification failure, the number of replicates with failure amplification from the total number of PCR replicates (%); ADO rate, the rate of allelic drop-out (%); FA rate, the rate of false alleles (%); and ER, overall error rate (%).