Figure 6.

VE-cadherin was transferred from ECs to cancer cells via EVs. (A) After 6 days of co-culturing MCF7-mCherry or MDA-MB-231-mCherry with GFP-labeled HUVECs, the extracellular vesicles were isolated from conditioned medium with Exo-spin column and added to MCF7 or MDA-MB-231 monoculture. White arrows showing CD63-GFP vesicles inside the breast cancer cells indicate the exosome transfer from HUVEC-GFP to cancer cells. (B) Incubation of MCF7 (upper panel) or MDA-MB-231 (lower panel) cells for 5 days with the EVs isolated from co-culture supernatant induced VE-cadherin (red) expression and its recruitment at the junctions (arrows) in breast cancer cells. (C,D) Line scan (red bars) from images of (B) quantifying the presence of VE-cadherin and GFP signal in the EVs derived from GFP-labeled HUVECs after being internalized by (C) MCF7 and (D) MDA-MB-231 cells.