Table 1.
Characteristics of included studies on diagnosis applications of aptamers in glioblastoma (GBM). Kd = dissociation constant.
| Author | Year | Aptamer | Nucleic Acid | Target | Tested | Kd | Conjugated Molecule | Diagnostic Application |
|---|---|---|---|---|---|---|---|---|
| Affinito et al. [19] | 2019 | A40s | 2′-fluoropyrimidine- RNA | In vitro; Ex vivo | 41.92 nM | miRNA; anti-miRNA; Cy5, Alexa488 | Confocal fluorescence microscope, histochemistry | |
| Fechter et al. [20] | 2019 | H02 | 2′-fluoropyrimidine- RNA | Integrin α5β1 | In vitro; Ex vivo | 72–277.8 nM | Cy5, Alexa564 | Confocal fluorescence microscope, SPR |
| Wu et al. [21] | 2018 | WYZ-41a WYZ-50a | DNA | A172 cells | In vitro | 75.27–168.56 nM | Cy5, FITC | Fluorescence microscope |
| Hasan et al. [22] | 2018 | Anti-EGFR | 2′-fluoropyrimidine- RNA | EGFRvIII | In vitro | Dynamic morphology | ||
| Mahmood et al. [23] | 2015 | Anti-EGFR | 2′-fluoropyrimidine- RNA | EGFRvIII | In vitro | Dynamic morphology | ||
| Alibolandi et al. [24] | 2014 | AS1411 | 2′-fluoropyrimidine- RNA | Nucleolin | In vitro | CdTe QDs | Fluorescence microscope | |
| Wu et al. [25] | 2014 | U2, U8, U19, U31 | DNA | EGFRvIII | in vivo; Ex vivo | 3.37–16.78 nM | 188RE | SPECT |
| Tan et al. [26] | 2013 | 32, 41, 43, 47 | DNA | EGFRvIII | In vitro | 0.62–37.57 nM | FITC, Biotin | Confocal fluorescence microscope, ELISA |
| Kim B. et al. [27] | 2013 | Anti-VEGFR2 | DNA | VEGFR2 | In vivo | 0.12 nM | carboxylated magnetic nanocrystal | MRI |
| Kim Y. et al. [28] | 2013 | A1, A2, A3, A4, A5 | DNA | TICs | In vitro | 0.12–3.75 nM | Cy3 | Fluorescence microscope |
| Li et al. [29] | 2013 | GB-10 | DNA | Tenascin-C | in vitro | 110 µM | NHS–PEG18–aldehyde | MR-FS |
| Kang et al. [30] | 2012 | GM128, GM131 | DNA | U118-MG cells | In vitro; Ex vivo | 20–37 nM | FAM | Confocal fluorescence microscope |
| Wan et al. [31] | 2011 | Anti-EGFR | 2′-fluoropyrimidine- RNA | EGFR and EGFRvIII | In vitro | Microfluidic device | ||
| Bayrac et al. [32] | 2011 | GMT3, GMT5, GMT9 | DNA | A172 cells | In vitro | 75.27–168.56 nM | Biotin | Fluorescence microscope |
| Wan et al. [33] | 2010 | Anti-EGFR | 2′-fluoropyrimidine- RNA | EGFR and EGFRvIII | In vitro | 2.4 nM | FAM | Fluorescence microscopy |
| Hicke et al. [34] | 2006 | TTA1 | 2′-fluoropyrimidine and 2′-OH purine RNA | Tenascin-C | In vivo | Rhodamine-RED, technetium-99m | Fluorescence microscopy, scintigraphy | |
| Daniels et al. [35] | 2003 | GB-10 | DNA | Tenascin-C | In vitro | 150 nM | Biotin | ELISA, SPR |
| Hicke et al. [36] | 2001 | TTA1 | 2′-fluoropyrimidine and 2′-OH purine RNA | Tenascin-C | In vivo | 3 nM | Phosphorus-32 | SPR |