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. 2020 Aug 8;12(8):2221. doi: 10.3390/cancers12082221

Figure 1.

Figure 1

FP assay setup and optimization with MSI1 protein and Numb RNA. (A) Binding of the RNA binding domain 1 (aa 20–107) of MSI1 (GB1-RBD1) to Numb RNA. GB1-RBD1 binds to NumbFITC RNA (5′-UAGGUAGUAGUUUUA-FITC-3′) but not to control oligo-FITC (CTLFITC RNA). The concentration of FITC-tagged RNA used in the assay was 2 nM (n > 3). (B) RNA competition assay. Increasing concentrations of unlabeled Numb or control RNAs were added to preformed Numb RNA-protein complexes. (C) Optimization of protein and RNA concentration in FP assay. (D) Effect of DMSO on the stability of the FP assay system. ns: not significant; * p < 0.05; *** p < 0.001 versus no DMSO control (concentration 0).