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Screening of libraries. (A) Library composition. Our MSI1/Numb mRNA FP-based screening assay was carried out with 1920 compounds from the NCI (Diversity Set II, natural product set, and approved oncology drugs) and in-house libraries. (B) Z’ score across plates. (C) Positive and negative controls’ values across plates. (D) Scattergram of the screening compounds. Median + 3SD was used as a threshold to pick the hits. Thirty-two hits were identified with a hit rate of 1.67%.
