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. 2020 Aug 8;12(8):2221. doi: 10.3390/cancers12082221

Figure 7.

Figure 7

Aza-9-liposome (Aza-9-lip) inhibits colon cancer cell proliferation. (A) MTT-based cytotoxicity assay of Aza-9-lip, free Aza-9, liposomes, and PBS in colon cancer cell line HCT-116. Aza-9 had no effect on the cell viability due to poor cellular uptake. (n = 3, one representative experiment of three is shown.) (B) PEGylated liposomes facilitate Aza-9 entry into the cells. Aza-9 fluoresces in the green channel. Images of Aza-9-lip (100 µM), Aza-9 (100 µM), and liposomes were taken at 2 h after the treatment. (C) Aza-9-lip inhibits HCT-116 and DLD-1 cell growth. (n = 3, ** p < 0.01; *** p < 0.001 versus liposomes control. One representative experiment of three is shown.).