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Absence of direct antibiotic effect of itaconic acid on PA14 wt biofilm grown in PPGAS medium for 24 h: biofilms were treated for 24 h with free itaconic acid (IA) 300 µg/mL. Cfu/mL values are depicted logarithmically for n = 3 experiments with technical triplicates each. Untreated PA14 wt biofilms served as controls. • represents mean value.
