Figure 2.

Reproducibility and diversity of splicing factor transcripts in cell-free saliva. For the reproducibility measurement, RNA was extracted from cell-free saliva from the same woman on different days. Diversity was measured by the extraction of RNA from ten different menstruating women with ages ranging from 20 y to 50 y (a) Real-time PCR was conducted for HNRNPA1, HNRNPA2B1, HNRNPA3, HNRNPK, HNRNPK exon 8 inclusion, PTBP1 and SRSF6 and normalized to PPIA. Relative expression levels were summed and plotted. (b) RNA from diversity and reproducibility groups was pooled and visualized on an Agilent bioanalyzer.