(A) Chemical structure of tricin. (B) UPLC chromatogram showing the peak of tricin detected at UV350 nm. (C) Photos of Hordei Fructus Germinatu, Oryzae Fructus Germinatus and Setariae Fructus Germinatus. (D) Effects of tricin on viability of HT29 and Colon26-Luc cells by MTT assay. Data were expressed as the mean percentage of the control (4 independent experiments with 4 replicates each, mean ± S.D.). (E,F) Effects of tricin on motility of (E) HT29 cells and (F) Colon26-Luc cells by scratch wound assay. Data were expressed as the mean percentage of untreated control (4 independent experiments with duplicate each, mean + S.D.). Statistical differences were determined by one-way ANOVA, followed by Tukey multiple comparisons test, with * p < 0.05, ** p < 0.01 against untreated control.