Figure 4.

Effects of tricin and FOLFOX on lung metastasis in orthotopic colon tumor-bearing mice. (A) Representative images of lungs obtained from different groups at end point of IVIS scan, and the fractional number revealed the frequency of metastasis detected in mice of each group. (B) Graph showed the bioluminescence measurements (expressed as average radiance) in lungs. Lungs were also collected and embedded in paraffin. Sections of lungs were stained with H & E and photographed. (C) Representative photos showed tumor nodules (yellow arrows) in lung sections. (D) Tumor burden were presented as the mean percentage of metastatic area. Mean + S.E.M., n = 7–13 in each group. Differences among treatment groups were determined by one-way ANOVA followed by Tukey’s multiple comparisons test, * p < 0.05 as compared with control group.