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. 2020 Jul 27;9(8):1789. doi: 10.3390/cells9081789

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ROS scavenger proteins SOD1 and PRDX5 are downregulated in Prm2-deficient sperm. (A) Profile plots showing the relative abundance of identified SOD1 peptides within each replicate of Prm2^+/+, Prm2^+/− and Prm2^−/− sperm. The protein abundance calculated by Tukey´s median polish is resembled by thickened grey lines. (B) Representative immunoblot against SOD1 and PRDX5 on protein lysates from Prm2^+/+, Prm2^+/− and Prm2^−/− testes and epididymal sperm (n = 1–3). Beta-actin and tubulin served as loading control.