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. 2020 Jul 27;9(8):1789. doi: 10.3390/cells9081789

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Secondary sperm defects are acquired during epididymal transit. (A) Periodic-acid-Schiff (PAS) staining of testicular tissue sections from Prm2^+/+, Prm2^+/− and Prm2^−/− males. Representative photomicrographs of stage VI/VII seminiferous tubules are shown. Proacrosomal vesicles of round spermatids are highlighted by arrowheads; mature acrosomes of step 16 spermatids by arrows. Scale bar: 20 μm. (B) Representative images of hematoxylin stained testicular step 16 spermatids. Scale bar: 25 µm. Quantification of sperm head length and width. Bars represent mean values ± SD (n = 5). (C) Representative images of DAPI-stained epididymal sperm from Prm2^+/+, Prm2^+/- and Prm2^-/- males. Scale bar: 20 µm. (D) Consensus shape computed for Prm2^+/+ (green), Prm2^+/− (orange) and Prm2^−/− (red) sperm populations.