Figure 8.

SST decreases pro-inflammatory markers induced by hypoxia and diabetic milieu in Bv.2 cells. (A) Effect of SST in the expression of pro-inflammatory parameters in hypoxia (3% O₂)-stimulated Bv.2 microglial cells. Bv.2 cells were pre-treated for 4 h with SST (10⁻⁶ M) and then cultured under hypoxia (3% O₂) for 24 h. mRNA levels of Tnfa, Il1b and Il6 were determined by RT-PCR. The results are means ± S.E.M (n = 5 independent experiments). * p ≤ 0.05 vs. basal normoxia condition; # p ≤ 0.05 vs. basal hypoxia condition (one-way ANOVA followed by Bonferroni t-test. (B) Effect of SST in the expression of pro-inflammatory markers in Bv.2 cells stimulated with 25 mM glucose + 300 µM H₂O₂ + 20 ng/mL IL-1β (referred as diabetic milieu: DM). Bv.2 microglial cells were pre-treated for 4 h with SST (10⁻⁶ M) and then cultured under the diabetic environment of the DM for a further 24 h. Tnfa, Il1b and Il6 mRNA levels were determined by RT-PCR. The fold change relative to the basal condition is shown (n = 3 independent experiments). * p ≤ 0.05 vs. diabetic milieu treatment (two-way ANOVA followed by Bonferroni t-test).