Table 1.
Independent variable levels, their combination, and the measured response for thymoquinone (TQ)–phytosome experimental runs prepared according to a Box-Behnken design.
| Run Number | Independent Variables | Dependent Variable | ||
|---|---|---|---|---|
| A (Mole/Mole) | B (°C) | C (h) | Y (nm) | |
| R1 | 1:3 | 50.00 | 2.00 | 80.7 |
| R2 | 1:3 | 65.00 | 1.00 | 54.9 |
| R3 | 1:3 | 50.00 | 2.00 | 82.2 |
| R4 | 1:3 | 50.00 | 2.00 | 81.7 |
| R5 | 1:3 | 65.00 | 3.00 | 64.7 |
| R6 | 1:3 | 35.00 | 1.00 | 112.8 |
| R7 | 1:3 | 35.00 | 3.00 | 114.7 |
| R8 | 1:1 | 50.00 | 3.00 | 71.8 |
| R9 | 1:3 | 50.00 | 2.00 | 82.2 |
| R10 | 1:5 | 50.00 | 3.00 | 101.8 |
| R11 | 1:5 | 65.00 | 2.00 | 69.7 |
| R12 | 1:1 | 35.00 | 2.00 | 98.8 |
| R13 | 1:5 | 50.00 | 1.00 | 95.2 |
| R14 | 1:5 | 35.00 | 2.00 | 129.9 |
| R15 | 1:1 | 65.00 | 2.00 | 43.7 |
| R16 | 1:3 | 50.00 | 2.00 | 79.8 |
| R17 | 1:1 | 50.00 | 1.00 | 60.4 |
| Independent variable | Levels | |||
| Low (−1) | Medium (0) | High (1) | ||
| (0) | (−1) | (+1) | ||
| TQ to phospholipid molar ratio (A) | 1:1 | 1:3 | 1:5 | |
| Process temperature (°C, B) | 35 | 50 | 65 | |
| Reflux time (h, C) | 1 | 2 | 3 | |
| Dependent variable | Goal | |||
| Vesicle Size (Y, nm) | Minimize | |||